Use of low-angle laser light scattering with gel permeation chromatography for the molecular mass determination of biomolecules.

Detection of serum proteins by high-pressure gel-permeation chromatography with low-angle laser light scattering, compared with analytical ultracentrifugation.

Human sera were subjected to analytical ultracentrifugation and "high-pressure" gel-permeation chromatography on a system of combined TSK Gel G5000 PW and G3000 SW columns. The chromatographic method produced remarkably superior resolution of the proteins, especially those exceeding 100 000 Da. We calculated the molecular masses of eluted fractions on the basis of their detection by low-angle l...

A Multiple Variable-Angle Light Scattering Detector for Gel Permeation Chromatography

A light scattering detector has been designed and constructed for use with gel permeation chromatographs. The detector measures light scattered from the eluting sample simultaneously at nine scattering angles, and is connected to a computer-controlled display which exhibits the angular dependence of the scattering in real time while the sample is eluting. Use of the light-scattering detector in...

Determination of the molecular mass of new L-fucose-containing glycosaminoglycan and its distribution by high-performance gel-permeation chromatography with laser light-scattering detection.

Gel permeation chromatography--multi-angle laser light scattering characterization of the molecular mass distribution of 'Pronova' sodium alginate.

510 Conclusions We have shown evidence for the bridging flocculation of PSL by p-LG. The change in surface charge of the PSL spheres resulting from adsorption of p-LG molecules correlates with the formation of aggregates bridged by protein molecules. To develop models of the kinetics of bridging flocculation, some measurements of aggregation as a function of time and temperature have been carri...

Determination of the absolute molecular weight averages and molecular weight distributions of alginates used as ice cream stabilizers by using multiangle laser light scattering measurements.

High-performance size exclusion chromatography with multiangle laser light scattering detection (HPSEC-MALLS) was used for characterizing complete molecular weight distributions for a range of commercial alginates used as ice cream stabilizers. For the samples investigated, molecular weight averages were found to vary between 115 000 and 321 700 g/mol and polydispersity indexes varied from 1. 5...